Biochemistry Seminar: Jonathan Coleman
Throughout the academic year, the Department of Biochemistry invites professors from other universities and institutes to speak at the University of Colorado Boulder. These seminars provide an opportunity for faculty and students to learn about exciting current research at other universities.
Come out and meet Dr. Jonathan Coleman during his seminar.
Dr. Coleman serves as a postdoctoral fellow at the Vollum Institute at Oregon Health and Science University.
This Seminar is hosted by the Department of Biochemistry. For more information about the seminar or Dr. Coleman's schedule, please email Dr. John Rinn.
Seminar Title: Structure and function of human SERT illuminates mechanism of alternating access in neurotransmitter sodium symporters
The serotonin transporter (SERT) mediates the sodium- and chloride-dependent reabsorption of the neurotransmitter into nerve terminals and is the target of psychostimulants and therapeutic agents. X-ray crystallographic studies show how antidepressants inhibit transport activity by ‘locking’ SERT in an outward-open conformation by binding to a central site. Occupancy of an allosteric site which resides at the periphery of the extracellular vestibule, sterically hinders ligand unbinding from the central site, providing an explanation for allosteric modulation of neurotransmitter sodium symporters. SERT-antibody complexes bound with ibogaine, a hallucinogenic natural product, were also investigated by single particle cryo-EM. Antibody complexes or the presence and absence of sodium, demonstrate how the transporter populates outward-open, occluded or inward-open conformations, respectively. Ibogaine binds to the central binding site and closure of the extracellular gate largely involves movements of transmembrane helices (TMs) 1b and 6a. Opening of the intracellular gate involves a hinge-like movement of TM1a and partial unwinding of TM5, which together create a permeation pathway enabling substrate and ion diffusion to the cytoplasm.
Wednesday, January 30 at 1:30pm to 2:30pm
Jennie Smoly Caruthers Biotec Building, Butcher Auditorium
3415 Colorado Avenue, Boulder, CO 80309